Expressed sequence tags from rhizomes of Glycyrrhiza uralensis

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Expressed sequence tags from rhizomes of Glycyrrhiza uralensis

Glycyrrhiza uralensis (Leguminosae, Fabaceae) is the most important medicinal plant used as a natural sweetener, in Chinese herbal medicines, and as a tobacco flavoring agent. Here, we report the generation of 56,857 expressed sequence tags (ESTs) derived from two full-length cDNA libraries produced from rhizomes (stolons) of two strains of G. uralensis, together with differential expression da...

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Antibacterial compounds from Glycyrrhiza uralensis.

From the roots of Glycyrrhiza uralensis, two new pterocarpenes, glycyrrhizol A (1) and glycyrrhizol B (2), along with four known isoflavonoids, 5-O-methylglycryol (3), isoglycyrol (4), 6,8-diisoprenyl-5,7,4'-trihydroxyisoflavone (5), and gancaonin G (6), were isolated using a bioassay-guided fractionation method. The structures of the new compounds (1and 2) were elucidated by spectroscopic data...

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Aldose reductase inhibitory compounds from Glycyrrhiza uralensis.

We evaluated the inhibitory effects of components from the root of Glycyrrhiza uralensis (G. uralensis) on aldose reductase (AR) and sorbitol formation in rat lenses with high levels of glucose as part of our ongoing search of natural sources for therapeutic and preventive agents for diabetic complications. In order to identify the bioactive components of G. uralensis, 5 prenylated flavonoids (...

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Analyses of expressed sequence tags from apple.

The domestic apple (Malus domestica; also known as Malus pumila Mill.) has become a model fruit crop in which to study commercial traits such as disease and pest resistance, grafting, and flavor and health compound biosynthesis. To speed the discovery of genes involved in these traits, develop markers to map genes, and breed new cultivars, we have produced a substantial expressed sequence tag c...

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Expressed Sequence Tags

Now a days, genome analysis has employed a rapid analysis tool known as expressed sequence tags. In 1983, SD Putney for the first time demonstrated the use of cDNA in identification of genome. The term expressed sequence tags (ESTs) was coined by Anthony Kerlavage at the Institute for Genomic Research. In 1991, Mark Adams used EST in relation to gene discovery and Human Genome project. cDNA or ...

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ژورنال

عنوان ژورنال: Plant Biotechnology

سال: 2009

ISSN: 1347-6114,1342-4580

DOI: 10.5511/plantbiotechnology.26.105